LEICA SP5 Confocal Microscope

LEICA SP5 Confocal Microscope


5x/NA 0.15 air
10x/NA 0.40 air
20x/NA 0.70 air
40x/NA 1.25 oil
63x/NA 1.40 oil

Laser lines

Diode – 405nm
Argon laser – 458, 476, 488 and 514nm laser lines
Diode – 561nm
HeNe – 633nm


3 standard (GaAs) PMTs
2 high sensitivity (HyD) PMTs
1 PMT for transmitted light
QImaging QICAM for widefield camera acquisition

Additional techniques

Incubator for live cell confocal imaging.
Argon laser for increasing contrast in thick samples or sequential bleach&scan FRAP imaging.
This system also provides a spectral module for λ scans and unmixing.