Facility

Multiscale Imaging

Multiscale Imaging

Andrea Doni

Head of Unit

The Multiscale Imaging Unit provides access to state-of-art light microscopy for scientists within and outside the institute, and performs independent and collaborative research for the development of advanced imaging-based technology.

The Multiscale Imaging Unit comprises a wide range of microscopes for live or fixed samples, including in vivo optical imaging instruments for luminescence and fluorescence tomography. High-capacity network of digital workstations is also available for the processing and analysis of imaging data.

The staff is involved in a wide range of scientific collaborations to support users in their studies in different fields of research, such as Oncology, Immunology, Neuroscience and Cardiology. The staff is also focused to the development of new methodologies and techniques aimed at significantly enhance bioimaging research.

major objectives of the Unit

🢒 Set up, maintain and develop optical technology for basic and advanced research

🢒 Provide theoretical and practical training to introduce researchers to optical and advanced imaging applications

🢒 Perform independent and collaborative research for the development of advanced imaging-based technology.

among our technologies

Bright-field microscopes

Wide-field fluorescence microscopes

Confocal microscopes

Super-Resolution (STED) mode

Single-Molecule Detection (SMD, FLIM/FCS) mode

Multi-photon microscope

Luminescence tomography

Fluorescence tomography

Metal-tagged Imaging Mass Cytometry

Machinery

We use high end technology of the latest industry standarts

Confocal Microscope FV1000
Confocal Microscope FV1000
LEICA SP8 Confocal Microscope (Basic)
LEICA SP8 Confocal Microscope (Basic)
LEICA SP8 Confocal Microscope (Advanced)
LEICA SP8 Confocal Microscope (Advanced)
LEICA SP5 Confocal Microscope
LEICA SP5 Confocal Microscope
LEICA SP8 STED3X SMD/FCS Confocal Microscope
LEICA SP8 STED3X SMD/FCS Confocal Microscope
Widefield CellR live cell imaging
Widefield CellR live cell imaging
LEICA DMi8 Wide-Field Microscope (Live Cell imaging)
LEICA DMi8 Wide-Field Microscope (Live Cell imaging)
IVIS Lumina III
IVIS Lumina III
FMT2000 (Fluorescence Tomography system)
FMT2000 (Fluorescence Tomography system)
Tissue optical clearing system
Tissue optical clearing system
Multiphoton Lavision Trimscope FLIM
Multiphoton Lavision Trimscope FLIM
Slide Scanner vs120 dotSlide
Slide Scanner vs120 dotSlide
Hyperion™ Imaging System
Hyperion™ Imaging System

Selected publications

Rasile M
EMBO J
Maternal immune activation leads to defective brain-blood vessels and intracerebral hemorrhages in male offspring.
Doni A
Nat Commun
Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus.
Medvedev R
Hypertension
Nanoscale Study of Calcium Handling Remodeling in Right Ventricular Cardiomyocytes Following Pulmonary Hypertension.
Erreni M
Biomolecules
Nanobodies as Versatile Tool for Multiscale Imaging Modalities.
Scott-Hewitt N
EMBO J
Local externalization of phosphatidylserine mediates developmental synaptic pruning by microglia.
Digifico E
Cancers (Basel)
Optimization of a Luciferase-Expressing Non-Invasive Intrapleural Model of Malignant Mesothelioma in Immunocompetent Mice.
Porta C
Cancer Res
Tumor-Derived Prostaglandin E2 Promotes p50 NF-ÎşB-Dependent Differentiation of Monocytic MDSCs.
Martini E
Circulation
Single-Cell Sequencing of Mouse Heart Immune Infiltrate in Pressure Overload-Driven Heart Failure Reveals Extent of Immune Activation.
Mattiola I
Nat Immunol
The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell-mediated resistance to metastasis.
Hall IF
Circ Res
Circ_Lrp6, a Circular RNA Enriched in Vascular Smooth Muscle Cells, Acts as a Sponge Regulating miRNA-145 Function.
Sobacchi C
Int J Mol Sci
3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells.
Filipello F
Immunity
The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity.
Miragoli M
Sci Transl Med
Inhalation of peptide-loaded nanoparticles improves heart failure.
Molgora M
Nature
IL-1R8 is a checkpoint in NK cells regulating anti-tumour and anti-viral activity.
Cambiaghi V
Endocrine
Identification of human somatostatin receptor 2 domains involved in internalization and signaling in QGP-1 pancreatic neuroendocrine tumor cell line.
Lodola F
Cell Death Dis
Adeno-associated virus-mediated CASQ2 delivery rescues phenotypic alterations in a patient-specific model of recessive catecholaminergic polymorphic ventricular tachycardia.
Strauss L
Cancer Cell
RORC1 Regulates Tumor-Promoting “Emergency” Granulo-Monocytopoiesis.
Doni A
J Exp Med
An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode.
Di Caro G
Clin Cancer Res
Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers.
Jaillon S
J Exp Med
The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps.

Facility Staff

Multiscale Imaging
Andrea Doni

Head of Unit

Multiscale Imaging
Marco Erreni

Technologist
Imaging scientist

Multiscale Imaging
Maria Rita Fumagalli

Data Scientist

Multiscale Imaging
Raffaella Parente

Technologist
Imaging scientist

Multiscale Imaging
Damiano Zanini

Technologist
Imaging scientist